Security is expected to be tight at Cambridge Crematorium, where preparations are underway for a private funeral at 7.30 p.m.
Hindley's body is expected to be driven the 30 miles from West Suffolk Hospital, where it has remained under police guard, to the crematorium on the outskirts of the city.
Fewer than 10 guests are expected to attend the private service, which officials have said will be as "dignified" as any other to allow her family to grieve.
Hindley's solicitor and the executor of her will, Andrew McCooey, who will be present at the service, says Hindley, a Roman Catholic, had requested a specific piece of music and a mass to be offered at her funeral.
The service in the small east chapel of the crematorium will be conducted by Father Michael Teader, Roman Catholic priest at Highpoint Prison. The cremated remains will then be handed to the Prison Service.
Hindley's relatives have asked for no flowers while Cambridge City Council says no extra crematorium staff are being drafted in, although stewards will help to manage the expected crowds of media outside the venue.
Hindley died at West Suffolk Hospital, Bury St Edmunds, last Friday aged 60 after falling ill at Highpoint Prison, Suffolk, where she had spent her final years.
An inquest which was opened and adjourned at the jail earlier this week heard she died of bronchial pneumonia caused by high blood pressure and damage to her coronary arteries.