Davids Lad's owners have accepted defeat in their courtroom action to allow their horse to run in next Saturday's Martell Cognac Grand National.
The four-strong Eddie Joe's syndicate made a last-ditch bid at the Supreme Court today to get the horse's 42-day imposed by the Turf Club lifted.
However, the judge decided to allow the suspension to stand pending a full hearing of the case.
The decision means the nine-year-old will not take his place in the line up at Aintree, and now looks likely to tackle either the Irish National at Fairyhouse or the attheraces Gold Cup at Sandown later in April.
Mattie Lynch, one of the syndicate, said: "We have had no joy so it's no Aintree for us this year.
"It was a once in a lifetime shot but I suppose it could be worse and now we'd be looking at the Irish Grand National or maybe the attheraces Gold Cup."
The long-running saga began when Davids Lad was hit with the ban after finishing last in the Paddy & Helen Cox Memorial Newlands Chase at Naas on February 23rd.
The nine-year-old was suspended from running after the Naas stewards decided that the racecourse had been used as a training ground, ruling him out of Aintree.
Jockey Timmy Murphy was banned for seven days and trainer Tony Martin fined 1,000. The horse's owners - brothers Eddie and Jimmy Moran, Nick Butterly and Lynch - took their case to court after their appeal against the suspension was turned down by the Turf Club's Appeals & Referrals Committee.
According to the Turf Club's lawyers, the 42 days run from the day after the date of the A & RC decision - so Davids Lad cannot run before April 15th.
The owners successfully made an ex-parte application to the High Court for a stay on the decision which was then challenged by the Turf Club, resulting in a two-day hearing at Dublin's High Court at which Justice Mella Carroll upheld the ban.
The syndicate must now decide whether to pursue a full hearing of the case but Denis Egan, chief executive of the Turf Club, was delighted by today's ruling.
He said: "We are thrilled. Hopefully we will get the same result at the full hearing.
"It will be going to a full hearing at a date in the future."
Davids Lad, who fell at the fourth last fence in last year's National when holding every chance, had been 14-1 second favourite for the Aintree marathon on April 5th.