Shooting Science - Trinity Biomedical Sciences contest Trinity Biomedical Sciences Institute celebrates its fifth birthday with a photography competition of work from their laboratories Sun 4 Sep 2016 Trinity Biomedical Sciences Institute celebrates its fifth birthday with a competition for the best images taken within their laboratories. A histological section of the native osteochondral tissue of the femoral condyle of a two-year-old goat. The sample was stained with hematoxylin and eosin and the image was taken under a polarised light microscope. Image: Pedro Diaz-Payno, PhD student at Trinity Centre for Bioengineering This image accompanies a study done into the comparison of the cell viability of 0.7mm thick nasal cartilage biopsies. The study aims to look at the cell viability before and after cryopreservation. Image: Megan Cloughley Transfection-induced cytoskeletal tension in mesenchymal stem cells. Fluorescence confocal photography. Image: Tomas Gonzalez-Fernandez, PhD candidate at Trinity Centre for Bioengineering New tissues generated by cells in a gel-like microenvironment. Various tissues in the human body degenerate with age or after injury. This includes damage of the extracellular matrix, including collagen fibres or other proteins around cells as well as cell death. Image represents molecular fluorescence staining of nucleus (using DAPI) and cytoskeleton (using Rhodamine Phalloidin) of the cell, which is a chondrocyte (cartilage cell) cultured in a fibrin hydrogel. Image: Jennifer Gansau, Trinity Centre for Bioengineering Cell surface glycome of differentiating neurons. Glycosylation is a ubiquitous modification of proteins and lipids in the central nervous system with roles in neural development and synaptic transmission. Here, we use synthetic azido-modified sugars and copper-click chemistry to image fully differentiated rat primary cortical neurons in high resolution with STED microscopy. Image: Darren O'Hara, Davey Lab, Trinity Biomedical Sciences Institute. Osteoclast formation. Confocal Microscopy- Fluorescent cholera toxin subunit B was used to stain the plasma membrane red and Hoechst was used to stain the nuclei blue. This allows the researcher to count the number of nuclei in the cell, as more nuclei indicate a more mature osteoclast. Image: Clare Cunningham/Gavin McManus, Microscopy and Imaging Facility, at Trinity Benzene Tricarboxamides: Designer soft material. Elucidating the structure of substances on the molecular and inter-molecular level is vital in order to understand and exploit the behaviour of functional materials in the bulk phase. A 3-dimensional structural model generated from single crystal X-ray diffraction data, overlaid on a hot-stage polarised optical microscopy photograph of a liquid-crystalline BTA derivative (top) and a scanning electron microscopy image of the BTA-derived gel fibres (bottom). Image: Dr Chris Hawes/Amy Lynes (Trinity) Edward Ward (Durham University) "This picture was taken a sunny morning in my lab, after I just had finished the synthesis of one of my compound. After evaporation of the solvent in the vial where the compound was collected, the result was this beautiful and unexpected motif. If we get closer we can imagine those lines to be small trees and we can actually get lost into this beautiful chemistry world." Image: Viola Previtali, PhD student in Isabel Rozas Group MicroRNA, a class of highly conserved post-transcriptional regulators, have been implicated in dysregulated angiogenesis. On such microRNA is miR-125, which we have shown to be associated with knee joint vascularity in psoriatic arthritis patients. To elucidate the functional role of miR-125 and angiogenesis in vivo we utilized Zebrafish to examine the complex formation of blood vessels in living animals. Technique: Confocal microscopy Cell on a G string - Live cell imaging of human neutrophils can illuminate the way in which they are activated by autoantibodies. In this image, an air bubble was inadvertently trapped in the chamber, causing the cells, which have been stained with a fluorescent dye, to palisade around it. Technique: Confocal microscopy. Image: Mark Little, Professor of Nephrology, Trinity Health Kidney Centre Interpenetrating network hydrogels. Hydrogels are mainly water, permeable, biocompatible and they degrade when cells inhabit them. The picture shows an interpenetrating network of two hydrogels that crosslink with UV-light. Histological slide of interpenetrating network stained with Safranin-O for polysaccharides and counterstained with Fast Green for proteins. Image: Dinorath Olvera, Trinity Centre for Bioengineering. Neurons and interneuronal processes projecting through the spinal cord of a postnatal mouse. 3-dimensional imaging of solvent cleared organs (iDisco) is a new histological technique based on lipid clearing from organs, enabling greater antibody penetration and image resolution. Imaging technique - Multiphoton microscopy - Image: Dr Denis Barry, Aisling O'Malley, Gavin McManus and Claire Simonie Phenotypic markers in the embryonic murine spinal cord. Growing axons (green) and radial glial cells (red) in the developing spinal cord. Imaging Technique - Epifluorescent microscopy. Image: Aisling O'Malley, Discipline of Anatomy, School of Medicine, Trinity College Dublin.