Iran's president said today the Jewish state was "illegitimate" and could not survive.
Mahmoud Ahmadinejad last year said that Israel should be "wiped of the map", echoing comments by the late founder of the Islamic Republic, Ayatollah Ruhollah Khomeini.
Iranian President Mahmoud Ahmadinejad
That remark caused an outcry in the West and he has not since repeated it.
But he regularly criticises Israel, a state that Iran has not recognised since the Islamic revolution in 1979.
"Our nation has previously announced that this regime is illegitimate from its foundation. It is fabricated. It has been imposed on the nations of the region and it cannot survive," he said in televised speech at a rally near Tehran.
Mr Ahmadinejad was speaking before Qods Day (Jerusalem Day), the last Friday of the Muslim holy month of Ramadan when Iranians show support for the Palestinians.
"The existence of this regime is the root of many problems of mankind today," the president said, adding that Israel had been "founded by the major powers in the heart of the Islamic world".
Mr Ahmadinejad's comments, which have included describing as a "myth" the Holocaust in which six million Jews were killed by the Nazis, have been condemned in Israel and the West.
Israeli Prime Minister Ehud Olmert said in May Iran's leaders had turned Israel "into a target for annihilation".
Defence Minister Shaul Mofaz said in April Iran's nuclear programme was the biggest threat to Jews since the Holocaust.